崇仁麻鸡小肠PepT1 mRNA基因差异表达

doi:10.11843/j.issn.0366-6964.2014.02.004

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (2): 191-196.doi: 10.11843/j.issn.0366-6964.2014.02.004

崇仁麻鸡小肠PepT1 mRNA基因差异表达

肖金华¹，武艳平²，何余湧¹，霍俊宏²，刘林秀²，季华员²，谢明贵²，康昭风²，谢金防^2*，陆伟^1*

（1.江西农业大学江西省高等学校动物营养与饲料重点实验室，南昌 330045；2.江西省农业科学院畜牧兽医研究所，南昌 330200）

收稿日期:2013-05-02 出版日期:2014-02-23 发布日期:2014-02-23
通讯作者: 谢金防，研究员，主要从事家禽育种研究，E-mail：xiejf166@163.com；陆伟，研究员，主要从事动物营养与饲料研究，E-mail：lw20030508@163.com
作者简介:肖金华(1988-)，女，江西余江人，硕士生，主要从事动物营养与饲料研究，E-mail：xjh0629@163.com
基金资助:
国家高技术研究发展计划项目（863）（2011AA100305）；江西省对外科技合作项目（2010EHB03800）；国家自然科学基金项目（31060296）；国家肉鸡产业技术体系项目（CARS-42-Z10）

Differential Expression of PepT1 mRNA in Small Intestine of Chongren Spotty Chicken

XIAO Jin-hua¹，WU Yan-ping²，HE Yu-yong¹，HUO Jun-hong²，LIU Lin-xiu²,JI Hua-yuan²，XIE Ming-gui²,KANG Zhao-feng²，XIE Jin-fang^2*，LU Wei^1*

(1.Key Laboratory of Animal Nutrition and Feed of Jiangxi Province，Jiangxi Agricultural University，Nanchang 330045， China； 2.Institute of Animal Husbandry and Veterinary Medicine，Jiangxi Academy of Agricultural Sciences，Nanchang 330200， China)

Received:2013-05-02 Online:2014-02-23 Published:2014-02-23

摘要/Abstract

摘要：

本研究旨在揭示崇仁麻鸡肠道PepT1 mRNA 的表达规律，为进一步研究小肽在肠道的吸收机理及其肽转运载体的表达分布情况提供基础。以120日龄崇仁麻鸡小肠肠道样品为模板，使用荧光定量PCR法研究肉鸡肠道寡肽转运载体1（Peptide transporter 1,PepT1）mRNA表达的肠段差异性以及十二指肠中高低饲料转化率组PepT1 mRNA的表达差异性。结果表明，崇仁麻鸡小肠PepT1 mRNA 的表达丰度从十二指肠到空肠再到回肠依次降低，其中在十二指肠的表达极显著的高于回肠（P<0.01），而十二指肠与空肠以及空肠与回肠之间差异不显著（P> 0.05）；在十二指肠中，高饲料转化率组PepT1 mRNA表达丰度要高于低饲料转化率组，但是差异不显著（P>0.05）。结果提示，PepT1 mRNA 主要是在崇仁麻鸡小肠的前段表达，十二指肠是PepT1 mRNA 表达的主要部位；饲料转化率高，其PepT1 mRNA的表达丰度也高。

Abstract:

This experiment was conducted to observe the expression of PepT1 mRNA in Chongren Spotty chicken intestine in order to support the study on the mechanism of transporting small peptide through small intestine and the distribution of peptide transporters.Samples were obtained from intestine of 120-day-old Chongren Spotty chicken and real-time fluorescent quantitative PCR was used to study the differential expression of peptide transporter 1 (PepT1) mRNA in different intestinal segments and breeds with different feed conversion rate.The results showed that the abundance of PepT1 mRNA gradually decreased from duodenum to jejunum and ileum，the expression of PepT1 mRNA in duodenum was significantly higher than that in ileum (P<0.01)，but there was no significant difference in the abundance of PepT1 mRNA between duodenum and jejunum or between jejunum and ileum (P>0.05).The abundance of PepT1 mRNA from breed with high feed conversion rate was higher than that from breed with low feed conversion rate (P>0.05).The results indicate that the anterior small intestine is the primary site for the expression of PepT1 mRNA and the abundance of PepT1 mRNA is the highest in the duodenum，meanwhile，Chongren Spotty chicken with high feed conversion rate has higher PepT1 mRNA abundance compared with those with low feed conversion rate.

中图分类号:

S831.2

肖金华，武艳平，何余湧，霍俊宏，刘林秀，季华员，谢明贵，康昭风，谢金防，陆伟. 崇仁麻鸡小肠PepT1 mRNA基因差异表达[J]. 畜牧兽医学报, 2014, 45(2): 191-196.

XIAO Jin-hua，WU Yan-ping，HE Yu-yong，HUO Jun-hong，LIU Lin-xiu,JI Hua-yuan，XIE Ming-gui,KANG Zhao-feng，XIE Jin-fang，LU Wei. Differential Expression of PepT1 mRNA in Small Intestine of Chongren Spotty Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 191-196.

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崇仁麻鸡小肠PepT1 mRNA基因差异表达

Differential Expression of PepT1 mRNA in Small Intestine of Chongren Spotty Chicken

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